Prepare the Matcha Mixture: In a small bowl, whisk together matcha powder and milk until smooth. Set aside.
Prepare the Strawberries: In a medium bowl, combine sliced strawberries with 1/4 cup of granulated sugar. Gently toss and let sit for 15 minutes, allowing the strawberries to macerate and release their juices.
Make the Mascarpone Cream: In a large bowl, whip heavy cream until soft peaks form. Gradually add remaining 1/4 cup of granulated sugar and vanilla extract. Continue whipping until stiff peaks form. In a separate bowl, beat mascarpone cheese until smooth. Gently fold whipped cream into mascarpone cheese until well combined.
Assemble the Tiramisu: Lightly dip each ladyfinger into the matcha mixture, ensuring they are moistened but not saturated. Arrange a layer of soaked ladyfingers in the bottom of a serving dish.
Layer the Ingredients: Spread half of the mascarpone cream over the ladyfingers. Top with half of the macerated strawberries, including some of the strawberry juices. Repeat layers with remaining ladyfingers, mascarpone cream, and strawberries.
Chill: Cover the tiramisu and refrigerate for at least 4 hours, or preferably overnight, to allow the flavors to meld and the ladyfingers to soften.
Serve: Before serving, dust the top of the tiramisu with powdered sugar, if desired.
Optional Tips: For a stronger matcha flavor, increase the amount of matcha powder in the matcha mixture. For a dairy-free option, use plant-based heavy cream and mascarpone cheese alternatives. A splash of almond extract can be added to the mascarpone cream for a complementary flavor.